Ultraviolet mutagenesis of radiation-sensitive (rad) mutants of the nematode Caenorhabditis elegans
Identifieur interne : 000897 ( Main/Exploration ); précédent : 000896; suivant : 000898Ultraviolet mutagenesis of radiation-sensitive (rad) mutants of the nematode Caenorhabditis elegans
Auteurs : Thomas Coohill [États-Unis] ; Tamara Marshall [États-Unis] ; Wayne Schubert [États-Unis] ; Gregory Nelson [États-Unis]Source :
- Mutation Research Letters [ 0165-7992 ] ; 1988.
Abstract
A mutational tester strain (JP10) of the nematode C. elegans was used to capture recessive lethal mutations in a balanced 300 essential gene autosomal region. The probability of converting a radiation interaction into a lethal mutation was measured in young gravid adults after exposure to fluences of 254-nm ultraviolet radiation (UV) ranging from 0 to 300 Jm−2. Mutation frequencies as high as 5% were observed. In addition, three different radiation-hypersensitive mutations, rad-1, rad-3 and rad-7 were incorporated into the JP10 background genotype, which allowed us to measure mutation frequencies in radiation-sensitive animals. The strain homozygous for rad-3 was hypermutable to UV while strains homozygous for rad-1 and rad-7 were hypomutable. Data showing the effects of UV on larval development and fertility for the rad mutants is also shown and compared for wild-type and JP10 backgrounds.
Url:
DOI: 10.1016/0165-7992(88)90025-5
Affiliations:
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The probability of converting a radiation interaction into a lethal mutation was measured in young gravid adults after exposure to fluences of 254-nm ultraviolet radiation (UV) ranging from 0 to 300 Jm−2. Mutation frequencies as high as 5% were observed. In addition, three different radiation-hypersensitive mutations, rad-1, rad-3 and rad-7 were incorporated into the JP10 background genotype, which allowed us to measure mutation frequencies in radiation-sensitive animals. The strain homozygous for rad-3 was hypermutable to UV while strains homozygous for rad-1 and rad-7 were hypomutable. Data showing the effects of UV on larval development and fertility for the rad mutants is also shown and compared for wild-type and JP10 backgrounds.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Kentucky</li></region></list><tree><country name="États-Unis"><region name="Kentucky"><name sortKey="Coohill, Thomas" sort="Coohill, Thomas" uniqKey="Coohill T" first="Thomas" last="Coohill">Thomas Coohill</name></region><name sortKey="Marshall, Tamara" sort="Marshall, Tamara" uniqKey="Marshall T" first="Tamara" last="Marshall">Tamara Marshall</name><name sortKey="Nelson, Gregory" sort="Nelson, Gregory" uniqKey="Nelson G" first="Gregory" last="Nelson">Gregory Nelson</name><name sortKey="Schubert, Wayne" sort="Schubert, Wayne" uniqKey="Schubert W" first="Wayne" last="Schubert">Wayne Schubert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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